Journal Article
Knödler, Matthias; Opdensteinen, Patrick; Sankaranarayanan, Ramya Ambur; Morgenroth, Agnieszka; Buhl, Eva Miriam; Mottaghy, Felix Manuel; Buyel, Johannes Felix
Simple plant-based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor-targeted drug delivery and bioimaging in cancer therapy
In: Biotechnology & bioengineering, 120 (2022), 4, 1038-1054
[DOI: 10.1002/bit.28312]

Journal Article
Gupta, Mainak Das; Flaskamp, Yannick; Roentgen, Robin; Juergens, Hannes; Armero-Gimenez, Jorge; Albrecht, Frank; Hemmerich, Johannes; Arfi, Zulfaquar Ahmad; Neuser, Jakob; Spiegel, Holger; Schillberg, Stefan Johannes; Yeliseev, Alexei; Song, Lusheng; Qiu, Ji; Williams, Charles; Finnern, Ricarda
Scaling eukaryotic cell-free protein synthesis achieved with the versatile and high-yielding tobacco BY-2 cell lysate
In: Biotechnology and Bioengineering, 120 (2023), 10, 2890-2906
[DOI: 10.1002/bit.28461]

Journal Article (Review Article)
Emonts, J.; Buyel, Johannes Felix
An overview of descriptors to capture protein properties - Tools and perspectives in the context of QSAR modeling
In: Computational and structural biotechnology journal, 21 (2023), 3234-3247
[DOI: 10.1016/j.csbj.2023.05.022]

Journal Article
Venkateswaran, Udhaya Pooranam; Caparco, Adam A.; González-Gamboa, Ivonne; Caballero, Reca Marian; Schuphan, Juliane; Steinmetz, Nicole F.
Plant Viral Nanocarrier Soil Mobility as a Function of Soil Type and Nanoparticle Properties
In: ACS Agricultural science & technology, 3 (2023), 7, 583-592
[DOI: 10.1021/acsagscitech.3c00074]

Journal Article
Knödler, Matthias; Reunious, Paul Winman; Buyel, Johannes Felix
Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter
In: BMC biotechnology, 23 (2023), 14
[DOI: 10.1186/s12896-023-00782-w]

Journal Article
Ridgley, Laura A.; Falci Finardi, Nicole; Gengenbach, Benjamin B.; Opdensteinen, Patrick; Croxford, Zack; Ma, Julian K.-C.; Bodman-Smith, Mark; Buyel, Johannes Felix; Teh, Audrey Y.-H.
Killer to cure: Expression and production costs calculation of tobacco plant‐made cancer‐immune checkpoint inhibitors
In: Plant biotechnology journal, 21 (2023), 6, 1254-1269
[DOI: 10.1111/pbi.14034]

Dissertation / PhD Thesis
Opdensteinen, Patrick
Assessment of a novel high-throughput process development platform for biopharmaceutical protein production (2023)
[DOI: 10.18154/RWTH-2023-04267]

Journal Article (Review Article)
Kouhen, Mohamed; García-Caparrós, Pedro; Twyman, Richard M.; Abdelly, Chedly; Mahmoudi, Henda; Schillberg, Stefan Johannes; Debez, Ahmed
Improving environmental stress resilience in crops by genome editing: insights from extremophile plants
In: Critical reviews in biotechnology, 43 (2022), 4, 559-574
[DOI: 10.1080/07388551.2022.2042481]

Dissertation / PhD Thesis
Kohl, Anna Christiane
Synthetic phenylpropanoid pathway for the in vivo production of coniferyl alcohol in Escherichia coli (2023)
[DOI: 10.18154/RWTH-2023-03313]

Journal Article
Bernau, C. R.; Buyel, J. F.
The use of antifoam agents to eliminate bubbles during biotechnological sample analysis
In: Discover chemical engineering, 3 (2023), 5
[DOI: 10.1007/s43938-023-00021-w]

Dissertation / PhD Thesis
Knödler, Matthias
Development and plant-based expression of recombinant immunotoxins for the targeted treatment of myelomonocytic leukemia (2023)
[DOI: 10.18154/RWTH-2023-01513]

Contribution to a book, Journal Article
Buntru, Matthias; Hahnengress, Nils; Croon, Alexander; Schillberg, Stefan Johannes
Plant-Derived Cell-Free Biofactories for the Production of Secondary Metabolites
In: Frontiers in plant science : FPLS, 12 (2022), 794999
[DOI: 10.3389/fpls.2021.794999]

Contribution to a book, Journal Article
Schiermeyer, Andreas; Cerda-Bennasser, Pedro; Schmelter, Thomas; Huang, Xin; Christou, Paul; Schillberg, Stefan
Rapid production of SaCas9 in plant‐based cell‐free lysate for activity testing
In: Biotechnology journal, 17 (2022), 7, 2100564
[DOI: 10.1002/biot.202100564]

Contribution to a book
Buntru, Matthias; Vogel, Simon; Finnern, Ricarda; Schillberg, Stefan Johannes
Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins
In: Recombinant proteins in plants : methods and protocols / edited by Stefan Schillberg and Holger Spiegel, Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany (2022), 113-124
[DOI: 10.1007/978-1-0716-2241-4_8]

Contribution to a conference proceedings, Journal Article
Spiegel, Holger; Nölke, Greta; Thangaraj, Harry; Schillberg, Stefan Johannes
The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use : A journey through a forest of regulatory and legal implications
In: Frontiers in Bioengineering and Biotechnology, 10 (2022), 926239
[DOI: 10.3389/fbioe.2022.926239]

Contribution to a book
Schillberg, Stefan Johannes; Spiegel, Holger
Recombinant Protein Production in Plants: A Brief Overview of Strengths and Challenges
In: Recombinant proteins in plants : methods and protocols / edited by Stefan Schillberg and Holger Spiegel, Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany (2022), 1-13
[DOI: 10.1007/978-1-0716-2241-4_1]

Contribution to a book
Spiegel, Holger; Schillberg, Stefan Johannes; Nölke, Greta
Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression
In: Recombinant proteins in plants : methods and protocols / edited by Stefan Schillberg and Holger Spiegel, Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany (2022), 89-102
[DOI: 10.1007/978-1-0716-2241-4_6]

Contribution to a book
Dickmeis, Christina; Commandeur, Ulrich Heinrich
Advanced Fusion Strategies for the Production of Functionalized Potato Virus X Virions
In: Recombinant proteins in plants : methods and protocols / edited by Stefan Schillberg and Holger Spiegel, Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany (2022), 215-239
[DOI: 10.1007/978-1-0716-2241-4_13]

Journal Article
Kauth, Louisa; Buhl, Eva Miriam; Luka, Julian; Richter, Karolin; Commandeur, Ulrich Heinrich; Dickmeis, Christina
Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles
In: Bio Tech, 11 (2022), 4, 49
[DOI: 10.3390/biotech11040049]

Journal Article
Steinmann, Andrea; Schullehner, Katrin; Kohl, Anna; Dickmeis, Christina; Finger, Maurice; Hubmann, Georg; Jach, Guido; Commandeur, Ulrich Heinrich; Girhard, Marco; Urlacher, Vlada B.; Lütz, Stephan
A targeted metabolomics method for extra- and intracellular metabolite quantification covering the complete monolignol and lignan synthesis pathway
In: Metabolic engineering communications, 15 (2022), e00205
[DOI: 10.1016/j.mec.2022.e00205]

Journal Article
Sheybanifard, Maryam; Guerzoni, Luis P. B.; Omidinia Anarkoli, Abdolrahman; De Laporte, Laura; Buyel, Johannes Felix; Besseling, Rut; Damen, Michiel; Gerich, Ad; Lammers, Twan; Metselaar, Josbert M.
Liposome manufacturing under continuous flow conditions: towards a fully integrated set-up with in-line control of critical quality attributes
In: Lab on a chip, 23 (2022), 1, 182-194
[DOI: 10.1039/D2LC00463A]

Contribution to a book, Journal Article (Review Article)
Bernau, Catherine Rose; Knödler, Matthias; Emonts, Jessica; Jäpel, Ronald Colin; Buyel, Johannes Felix
The use of predictive models to develop chromatography-based purification processes
In: Frontiers in Bioengineering and Biotechnology, 10 (2022), 1009102
[DOI: 10.3389/fbioe.2022.1009102]

Contribution to a conference proceedings, Journal Article (Editorial)
Buyel, Johannes Felix; Benvenuto, E.; Meyers, A. E.
Editorial: Proceedings of the 4th biennial conference of the International Society for Plant Molecular Farming
In: Frontiers in Bioengineering and Biotechnology (2022), 1023227
[DOI: 10.3389/fbioe.2022.1023227]

Contribution to a book, Journal Article
Hou, Hsuan-Wu; Bishop, Christopher A.; Huckauf, Jana; Broer, Inge; Klaus, Susanne; Nausch, Henrik; Buyel, Johannes Felix
Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis
In: Frontiers in plant science : FPLS, 13 (2022), 998596
[DOI: 10.3389/fpls.2022.998596]

Journal Article
Jäpel, Ronald Colin; Buyel, Johannes Felix
Bayesian optimization using multiple directional objective functions allows the rapid inverse fitting of parameters for chromatography simulations
In: Journal of chromatography / A, 1679 (2022), 463408
[DOI: 10.1016/j.chroma.2022.463408]

Dissertation / PhD Thesis
Beuel, Ann-Katrin
LEDitGROW - Beleuchtungssysteme zur Optimierung von Pflanzenzellkulturen für die Sekundärmetabolit-Produktion (2022)
[DOI: 10.18154/RWTH-2022-09555]

Dissertation / PhD Thesis
Pietschmann, Jan
Nanosonden-basierte Mykotoxin-Detektion in Agrarprodukten (2022)
[DOI: 10.18154/RWTH-2022-07922]

Journal Article
Opdensteinen, Patrick; Sperl, Laura E.; Mohamadi, Mariam; Kündgen-Redding, Nicole; Hagn, Franz; Buyel, Johannes Felix
The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy
In: Plant biotechnology journal, 20 (2022), 10, 1928-1939
[DOI: 10.1111/pbi.13873]

Journal Article
Opdensteinen, P.; Buyel, Johannes Felix
Reducing water uptake into BY-2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs
In: Biotechnology journal : BTJ, 17 (2022), 10, 2200134
[DOI: 10.1002/biot.202200134]

Journal Article
Frank, K.; Bernau, C. R.; Buyel, Johannes Felix
Spherical nanoparticles can be used as non-penetrating tracers to determine the extra-particle void volume in packed-bed chromatography columns
In: Journal of chromatography / A, 1675 (2022), 463174
[DOI: 10.1016/j.chroma.2022.463174]

Journal Article
Biancacci, Ilaria; De Lorenzi, Federica; Theek, Benjamin; Bai, Xiangyang; May, Jan-Niklas; Consolino, Lorena; Baues, Maike; Moeckel, Diana; Gremse, Felix; Stillfried, Saskia; El Shafei, Asmaa; Benderski, Karina; Azadkhah Shalmani, Armin; Wang, Alec; Momoh, Jeffrey; Peña, Quim; Buhl, Eva Miriam; Buyel, Johannes Felix; Hennink, Wim; Kießling, Fabian; Metselaar, Josbert; Shi, Yang; Lammers, Twan
Monitoring EPR Effect Dynamics during Nanotaxane Treatment with Theranostic Polymeric Micelles
In: Advanced science (2022), 2103745
[DOI: 10.1002/advs.202103745]

Abstract, Contribution to a conference proceedings, Journal Article
Lin, Ying-Ying; Schuphan, Juliane; Dickmeis, Christina; Buhl, Eva Miriam; Commandeur, Ulrich Heinrich; Fischer, Horst
Ultralow Amount of Engineered Plant Viral Nanoparticles Attached on Mesenchymal Stem Cells Enhances Osteogenesis and Mineralization
In: Tissue engineering / A, 28 (2022), Supplement 1, 483

Patent
Stein, Christoph; Mladenov, Radoslav; Stockmeyer, Bernhard; Barth, Stefan; Schenke, Lea Christin; Fischer, Rainer
Anti-CD89 zytotoxische Komplexe (2021)

Patent
Barth, Stefan; Hussain, Ahmad Fawzi; Fischer, Rainer
Specific photoimmuno-theranostics for detection and elimination of skin cancer cells (2021)

Journal Article
Ma, Yifeng; Commandeur, Ulrich Heinrich; Steinmetz, Nicole F.
Three Alternative Treatment Protocols for the Efficient Inactivation of Potato Virus X
In: ACS applied bio materials, 4 (2021), 12, 8309-8315
[DOI: 10.1021/acsabm.1c00838]

Dissertation / PhD Thesis
Bernau, Catherine Rose
Model-based and rational design of plant purification processes (2021)
[DOI: 10.18154/RWTH-2022-00942]

Journal Article
Opdensteinen, Patrick; Dietz, S. J.; Gengenbach, Benjamin Bruno; Buyel, Johannes Felix
Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms
In: Frontiers in Bioengineering and Biotechnology, 9 (2021), 708150
[DOI: 10.3389/fbioe.2021.708150]

Contribution to a book
Fischer, Rainer; Holland, Tanja; Sack, Markus; Schillberg, Stefan; Stoger, Eva; Twyman, Richard M.; Buyel, Johannes Felix
Glyco-Engineering of Plant-Based Expression Systems
In: Advances in Glycobiotechnology / edited by Erdmann Rapp, Udo Reichl (2018)
[DOI: 10.1007/10_2018_76]

Journal Article
Schuphan, Juliane; Commandeur, Ulrich Heinrich
Analysis of Engineered Tobacco Mosaic Virus and Potato Virus X Nanoparticles as Carriers for Biocatalysts
In: Frontiers in Functional Plant Ecology, 12 (2021), 710869
[DOI: 10.3389/fpls.2021.710869]

Contribution to a book, Journal Article (Preface)
Christou, Paul; Dhingra, Amit; Slamet-Loedin, Inez H.; Oliveira, Margarida; Chakraborty, Supriya; Buyel, Johannes Felix; Stoger, Eva; Schillberg, Stefan; Orzaez, Diego; Quemada, Hector
Preface: Genome editing in plants
In: Transgenic research, 30 (2021), 4, 317-320
[DOI: 10.1007/s11248-021-00268-5]

Journal Article (Review Article)
Lobato Gómez, Maria; Huang, Xin; Alvarez, Derry; He, Wenshu; Baysal, Can; Zhu, Changfu; Armario-Najera, Victoria; Blanco Perera, Amaya; Cerda Bennasser, Pedro; Saba-Mayoral, Andera; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes Felix; da Cunha, Nicolau B.; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyappan; Häkkinen, Suvi T.; Kumar, Shashi; Ramalingam, Sathish Kumar; Lacorte, Cristiano; Lomonossoff, George P.; Luís, Ines M.; Ma, Julian K.-C.; McDonald, Karen A.; Murad, Andre; Nandi, Somen; O’Keefe, Barry; Oksman-Caldentey, Kirsi-Marja; Parthiban, Subramanian; Paul, Mathew J.; Ponndorf, Daniel; Rech, Elibio; Rodrigues, Julio C. M.; Ruf, Stephanie; Schillberg, Stefan; Schwestka, Jennifer; Shah, Priya S.; Singh, Rahul; Stoger, Eva; Twyman, Richard M.; Varghese, Inchakalody P.; Vianna, Giovanni R.; Webster, Gina; Wilbers, Ruud H. P.; Capell, Teresa; Christou, Paul
Contributions of the international plant science community to the fight against human infectious diseases : part 1: epidemic and pandemic diseases
In: Plant biotechnology journal, 19 (2021), 10, 1901-1920
[DOI: 10.1111/pbi.13657]

Contribution to a book, Journal Article
Augustine, Sruthy Maria; Cherian, Anoop Vadakan; Seiling, Kerstin; di Fiore, Stefano; Raven, Nicole; Commandeur, Ulrich Heinrich; Schillberg, Stefan
Targeted mutagenesis in Nicotiana tabacum ADF gene using shockwave-mediated ribonucleoprotein delivery increases osmotic stress tolerance
In: Physiologia plantarum, 173 (2021), 3, 993-1007
[DOI: 10.1111/ppl.13499]

Journal Article (Review Article)
He, Wenshu; Baysal, Can; Lobato Gómez, Maria; Huang, Xin; Alvarez, Derry; Zhu, Changfu; Armario-Najera, Victoria; Blanco Perera, Aamaya; Cerda Bennaser, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Alexandra Abreu, Isabel; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes Felix; Cunha, Nicolau B.; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyappan; Häkkinen, Suvi T.; Kumar, Shashi; Sathish Kumar, Ramalingam; Lacorte, Cristiano; Lomonossoff, George P.; Luís, Ines M.; K.-C. Ma, Julian; McDonald, Karen A.; Murad, Andre; Nandi, Somen; O'Keef, Barry; Parthiban, Subramanian; Paul, Mathew J.; Ponndorf, Daniel; Rech, Elibio; Rodrigues, Julio C. M.; Ruf, Stephanie; Schillberg, Stefan; Schwestka, Jennifer; Shah, Priya S.; Singh, Rahul; Stoger, Eva; Twyman, Richard M.; Varghese, Inchakalody P.; Vianna, Giovanni R.; Webster, Gina; Wilbers, Ruud H. P.; Christou, Paul; Oksman-Caldentey, Kirsi-Marja; Capell, Teresa
Contributions of the international plant science community to the fight against infectious diseases in humans - part 2: Affordable drugs in edible plants for endemic and re‐emerging diseases
In: Plant biotechnology journal, 19 (2021), 10, 1921-1936
[DOI: 10.1111/pbi.13658]

Journal Article
Bernau, C. R.; Jäpel, R. C.; Hübbers, Jan Wilhelm; Nölting, S.; Opdensteinen, P.; Buyel, Johannes Felix
Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins
In: Journal of chromatography / A, 1652 (2021), 462379
[DOI: 10.1016/j.chroma.2021.462379]

Journal Article
Popović, Nikolina; Pržulj, Dunja; Mladenović, Maja; Prodanović, Olivera; Ece, Selin; Ilić Đurđić, Karla; Ostafe, Raluca; Fischer, Rainer; Prodanović, Radivoje
Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization
In: International journal of biological macromolecules, 181 (2021), 1072-1080
[DOI: 10.1016/j.ijbiomac.2021.04.115]

Contribution to a book, Journal Article
Menghiu, Gheorghita; Ostafe, Vasile; Prodanović, Radivoje; Fischer, Rainer; Ostafe, Raluca
A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A
In: International journal of molecular sciences, 22 (2021), 6, 3041
[DOI: 10.3390/ijms22063041]

Dissertation / PhD Thesis
Gengenbach, Benjamin Bruno
Rational and model-based characterization of heterologous gene expression in biological systems (2021)
[DOI: 10.18154/RWTH-2021-04516]

Journal Article
Knödler, Matthias; Buyel, Johannes Felix
Plant-made immunotoxin building blocks : A roadmap for producing therapeutic antibody-toxin fusions
In: Biotechnology advances, 47 (2020), 107683
[DOI: 10.1016/j.biotechadv.2020.107683]

Journal Article
Nausch, Henrik; Buyel, Johannes Felix
Cryopreservation of plant cell cultures : Diverse practices and protocols
In: New biotechnology, 62 (2021), 86-95
[DOI: 10.1016/j.nbt.2021.02.002]

Contribution to a book, Journal Article
Buyel, Johannes Felix; Stöger, E.; Bortesi, L.
Targeted genome editing of plants and plant cells for biomanufacturing
In: Transgenic research, 30 (2021), 4, 401-426
[DOI: 10.1007/s11248-021-00236-z]